Affinity electrophoresis in gels containing hydrophobic substituents.

Electrophoresis of hydrophobic proteins

The electrophoresis of hydrophobic proteins underwent signi®cant development during the last decade. In native gel electrophoresis, the induced charge shift, the involvement of mild detergents as well as a constant ionic strength in the electrophoresis, overcame the dif®culty of the low solubility and aggregation of the hydrophobic proteins, and critically improved the results of their native P...

Processing of 2D Electrophoresis Gels

Here we present results on 2D electrophoresis gel image processing using methods that provide a measure of meaningfulness. This work is part of an ongoing project on biomedical image analysis. Biomedical sciences have a long tradition in our country and therefore there is extensive experience in this area. Unfortunately, economical restrictions sometimes do not allow our researchers to have the...

Automated detection of point mutations by electrophoresis in peptide-nucleic acid-containing gels.

Polymerization of electronically, essentially neutral peptide nucleic acids (PNA) into polyacrylamide gels creates a medium in which the salt-independent properties of PNA/DNA interactions are used to achieve hybridization with target DNA during affinity electrophoresis. Such physical entrapment of PNA has been used to differentiate between a retarded, complementary DNA strand and a non-retarde...

Cavity-containing supramolecular gels as a crystallization tool for hydrophobic pharmaceuticals.

We present two approaches to low-molecular-weight supramolecular gels bearing hydrophobic cavities based on calixarene-containing building blocks. Gels are formed by a calixarene based tetrahydrazide gelator or a co-gel of a calixarene diammonium salt and a bis-crown ether. The calixarene hydrophobic cavity enables the complexation of hydrophobic drug molecules in a generic fashion thus providi...

Affinity Capillary Electrophoresis